As of Episode XCV, only Ashi was confirmed to be alive. They are from a cult of the same name dedicated to Aku and appear during the events of Season 5. The Daughters of Aku were a group of seven human-demon hybrid women who were born and raised by their mother, the High Priestess, for the sole purpose of killing Samurai Jack.
Samurai Jack (brother-in-law, alternate timeline)
